12917107 (Tissue: lens) Previous pharmacological evaluation of these sheep lens cultures indicated the presence of purinergic receptors ( 6 ); therefore, it was suspected that ATP activated a purinergic receptor-signaling pathway resulting in the activation of PLC generating IP3 and diacylglycerol.
11171588 As reported previously ( 1 ) an inhibitor of phospholipase C (PLC), U-73122 (2.5 أƒآƒأ‚آ¯أƒآ‚أ‚آ؟أƒآ‚أ‚آ½M), which inhibits IP3 production, significantly reduced spontaneous Ca2+ transients ( P < 0.05).
10199807 (Tissue: epithelia, CellLineName: cf) The P2Y2 receptor is a G protein-coupled receptor that activates the phospholipase C-dependent generation of inositol 1,4,5-trisphosphate and intracellular Ca2+ mobilization ( 5 , 35 ).
9973202 (CellLineName: H345) Binding of bombesin to its receptor in H345 cells is known to induce phospholipase C-mediated formation of the second messengers DAG and inositol 1,4,5-trisphosphate, which activate classic and novel isoforms of PKC and mobilize Ca 2+ , respectively (8 , 34 , 35) .
10706116 Activated phospholipase C metabolizes phosphatidylinositol bisphosphate to produce diacylglycerol and inositol 1,4,5-trisphosphate (7 , 8) .
16760378 (Organ: lung) PLC activation induces protein kinase C (PKC) activation and inositol 1,4,5-trisphosphate (IP 3 ) generation, which serve to increase intracellular Ca 2+ and sensitize and activate the cell's contractile machinery [ 19 , 20 ].
12193412 (Organ: renal) Activation of phospholipase C increases the hydrolysis of PIP 2 to produce diacylglycerol and inositol 1,4,5-trisphosphate (IP 3 ).
9569230 (Tissue: glia) According to this model, mechanical stimulation of a cell activates both Ca2+-conducting channels, which allow the influx of extracellular Ca2+, and PLC, which generates IP3 in the stimulated cell.