14688255 This co-expression system was used previously in our laboratory to activate GST-RSK CTD ( 21 ), and ERK2 has been reported to activate MK2 ( 26 ).
8995385 (CellType: leukocyte) First, LY294002, which inhibited Erk-1 and 2 but not p38, failed to inhibit MAPKAPK-2.
8900202 (CellLineName: Pam 212) MBP is an efficient substrate for ERK1 and ERK2, whereas GST-cJun (1-169) includes the activating phosphorylation sites Ser-63 and Ser-73, which have been shown to be phosphorylated by SAPK/JNK but not by ERK1, ERK2, or p38 HOG1 ( 3 ). 2 Similarly, hsp27 is an efficient substrate for MAPKAPK2, which in turn is predominantly activated by p38 HOG1, although MAPKAPK2 can also be phosphorylated in vitro by ERK1 and ERK2 ( 29 , 30 ). -- Fig. 2.
14499342 (CellType: neutrophil) ERK phosphorylated MK2 in vitro and activated MK2 in f-methionyl-leucyl-phenylalanine (FMLP)-stimulated neutrophils.
8846784 (CellLineName: KB) In contrast, full-length GST-MAPKAP kinase-2 was phosphorylated at Thr25 (and not activated) by p42 MAPK, suggesting a role for the N-terminal domain in specifying activation by RK in vivo.
10625698 (Organ: ventricle, CellType: myocyte) Activated MAPK interacts with a number of downstream targets, including the p90 ribosomal S6 kinase (p90 Rsk ) ( 19 ) and MAPK-activated protein kinase-2 (MAPKAP-K2 ) ( 20 , 21 ), which are activated by ERK1/2 and p38 MAPK , respectively.
11463795 (Tissue: skeletal muscle, CellType: adipocyte) With respect to alternative non-PLD/PA mechanisms whereby ERK could conceivably activate PKC-eta/ , it has been reported that ERK-activated MAPK-activated kinase-2 ( 34 , 35 ) and MSK-1 ( 36 ) can act like PDK-1 and/or PDK-2 and phosphorylate PKB on serine 308 and threonine 473 residues.