Unlike wild-type worms, atfs-1(tm4525) (deletion mutation of atfs-1) animals were unable to induce hsp-60pr::gfp when raised on spg-7(RNAi), a mitochondrial protease required for ETC quality control and mitochondrial ribosome biogenesis. atfs-1(tm4525) knockout mutants failed to induce expression of endogenous hsp-60 in response to spg-7 RNAi, as determined by RT-PCR.