In addition, an interaction between synphilin-1 and HDAC6 was observed
The precipitates were probed with HA antibodies to detect HDAC6 and revealed an interaction of HDAC6 with both kalirin-7 and synphilin-1. 30 mg of protein lysates were visualized as input control.
(B) When kalirin-7 is overexpressed, it facilitates the recruitment of HDAC6 and the dynein motor complex and acts on microtubule dynamics by stimulating the deacetylase activity of HDAC6, thereby increasing the transportation of synphilin-1 into aggresomes.