The physical interaction between septins and HDAC6 is consistent in three distinct biochemical assays; first, co-immunoprecipitation
of native SEPT7 and HDAC6 from extracts of
cerebrocortical neurons; second, co-immunoprecipitation of
SEPT7 and HDAC6 co-expressed in nonneural cells; and finally,beads-capture assay with a pure recombinant SEPT7/6/2 complex and HDAC6.
SEPT7 facilitates HDAC6/acetylated a-tubulin interactiond that septins/HDAC6-mediated deacetylation of microtubules
is the common mechanism that couples septins and the dynamic growth of microtubules, shared by the dendrites and axons of sprouting neurons.