HDAC6 also forms a complex with protein phosphatase 1 (PP1). Dissociation of the complex induced by the HDAC inhibitor trichostatin A (TSA) results in increased PP1-Akt association and decreased Akt phosphorylation.
To investigate the direct association of PP1 with HDACs, we undertook Far-Westerns of purified hexahistidine-tagged HDAC6 and -4 proteins using digoxigenin-conjugated PP1α.
Note: PP-1C has several subunits, based on the information from the origical article(The third reference,Brush MH et al, 2004), subunit alpha is demonstared here in the network.