8626650 Here we report that mutations within the switch 2 domain of Ras (residues 62-69) inhibit activation of Ras by the mammalian GEFs, Sos1, and GRF/CDC25Mm.
1447167 As a consequence, the CDC25Mm induces a rapid formation of the biologically active Ras.GTP complex.
(Tissue: blood, CellType: b cell) Phosphorylation of RasGRP3 by PKC may be critical to modulate its association with Ras at the plasma membrane and the subsequent activation of the ERK MAPK cascade.
14749369 Furthermore, we have recently demonstrated that the ability of RasGRF1 to activate Ras is regulated by the Rho family GTPase Cdc42, by a mechanism that entails the translocation of RasGRF1 to the cell particulate fraction ( 3 , 4 ).
14676298 (CellType: b cell) This defective Ras activation is suppressed by the expression of RasGRP3 as a membrane-attached form, suggesting that phospholipase C-gamma2 regulates RasGRP3 localization and thereby Ras activation.
11500497 Our results suggest that mUBPy may play a role in controlling degradation of CDC25 Mm , thus regulating the level of this Ras-guanine nucleotide exchange factor.
15572660 (Tissue: endothelial) However, RasGRP2 is a Rap1 activator and not a Ras activator, whereas RasGRP3 is an activator of Ras and Rap1.
15213298 RasGRP3 activates both Ras and Rap1 (Yamashita et al., 2000 ), and RasGRP4 activates Ras (Reuther et al., 2002 ; Yang et al., 2002 ).
8717044 (CellLineName: COS-7) These results demonstrate a G-protein-coupled mechanism for Ras activation, mediated by p140 Ras-GRF.
8700529 (CellLineName: CHO) Transient expression of CDC25Mm in CHO cells activates Ras.