10713965 PDBu-induced activation of PKC promotes the priming of secretory granules, thereby enhancing the efficacy for Ca2+ to trigger fusion/exocytosis.
8429046 It appears, therefore, that the binding of Ca2+ to PKC requires a complex tertiary structure in the regulatory domain.
1313322 (Tissue: articular cartilage, CellType: chondrocyte) The activity of various subspecies of protein kinase C (PKC) in chondrocytes derived from rodent costal cartilage and bovine articular cartilage has been determined and the role of PKC in GAG synthesis as well as the possible interactions of PKC with calcium- or cyclic AMP (cAMP)-dependent systems in the synthesis of GAG.
10325235 (CellType: cardiomyocyte) Phorbol ester-sensitive PKC isoforms were detected at very low levels in caveolae fractions prepared from unstimulated cardiomyocytes; phorbol 12-myristate 13-acetate (PMA) (but not 4alpha-PMA, which does not activate PKC) recruited calcium-sensitive PKCalpha and novel PKCdelta and PKCepsilon to this compartment.
9725212 (CellType: t cell) Since cyclosporin does not have any effect on cytosolic Ca 2+ levels and thus does not interfere with the interaction between PKC and Ca 2+ , we tested the effect of an inhibitor of intracellular Ca 2+ redistribution, TMB-8 ( 36 ), on TCR cross-linking-induced AICD.
10751227 (Tissue: mesangia) DAG can directly activate classical types of PKC by interacting with its lipid-binding domain, and IP3 can indirectly activate PKC by increasing intracellular Ca2+, which interacts with the PKC Ca2+-binding domain.
11342594 Synaptotagmins are transmembrane proteins with a short NH2-terminal ectodomain, a single transmembrane region, and two highly conserved, independently folding Ca2+-binding C2 domains (C2A and C2B) homologous to the C2 regulatory region of protein kinase C.
7836388 At odds with the concept of calcium modulation of lipid binding, these authors considered phospholipids as modulators for the binding of calcium to PKC( 31 , 34 ) .
9852158 The four inhibitors of PKC all accelerated the breakdown of the F-actin network observed after 5 min (Fig. 6 D ) and 30 min (Fig. 6 E ) in 0 Ca 2+ .
9218480 These data reveal that the negative charge on the carboxyl terminus of protein kinase C contributes to the interaction of protein kinase C with Ca 2+ .