10026206 Use of the specific MEK1/2 inhibitor, PD98059, which prevents Raf-mediated activation of MEK1/2 ( 42 ), allowed us to evaluate the role of the MEK/ERK pathway in CD40-mediated induction of IL-1eta and TNFalpha synthesis.
11124968 (CellType: promyelocyte) PMA-induced ERK activation is through a kinase cascade that requires Raf-1-dependent phosphorylation of MEK1/2 on serines 217 and 221 and MEK1/2-dependent phosphorylation of ERK1/2 on threonine 202 and tyrosine 204 ( 26 , 27 ).
7499206 PD 098059 inhibited the activation of MAPKK2 by Raf with a much higher IC value (50 أƒآƒأ‚آ¯أƒآ‚أ‚آ؟أƒآ‚أ‚آ½M) and did not inhibit the phosphorylation of other Raf or MEK kinase substrates, indicating that it exerts its effect by binding to the inactive form of MAPKK1.
11296227 (CellLineName: Sf9) Immunoprecipitated endogenous Raf-1 can phosphorylate MEK1 and -2 in vitro ( Howe et al ., 1992 ; Kyriakis et al ., 1992 ; Marais et al ., 1998 ) and the Raf/MEK/ERK cascade can be reconstituted in vitro using proteins expressed in Sf9 cells ( Macdonald et al ., 1993 ).
7797459 (CellType: fibroblast, CellLineName: PC 12) MEK1 and -2 are activated not only by Raf-1 and B-Raf ( 19 , 20 ) but also by the Mos protooncogene product( 21 , 22 ) , MEK kinase 1 (MEKK1) ( ) ( 23 ) , and other probably distinct, growth factor-stimulated activities( 24 , 25 ) .